lncrna + mrna human gene expression microarray v4.0 platform  (Agilent technologies)

Journal: Reviews in Cardiovascular Medicine

Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

doi: 10.31083/j.rcm2407214

Figure Lengend Snippet: The regulatory mechanism of the competitive endogenous RNA networks in transcription, translation, and epigenetics . LncRNA regulates transcription by splicing and degrading RNA, while posttranscriptional regulation is controlled by decoy and sponge proteins, as well as epigenetic modification. CircRNA regulates transcription and translation by binding to miRNA and interacting with RNA-binding protein, particularly in certain circRNA can code protein. Both lncRNA and circRNA can serve as competitive endogenous RNA that bind with miRNA to regulate the translation of targeted mRNA, thereby inhibiting mRNA translation and promoting mRNA degradation. See text for details. circRNA, circular RNA; lncRNA, long non-coding RNA; miRNA, microRNA; mRNA, messenger RNA; UTR, untranslated regions.

Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

Techniques: Modification, Binding Assay, RNA Binding Assay

Journal: Reviews in Cardiovascular Medicine

Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

doi: 10.31083/j.rcm2407214

Figure Lengend Snippet: The lncRNA associated ceRNA networks in sepsis-induced cardiotoxicity .

Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

Techniques: Biomarker Discovery, Diagnostic Assay, Activity Assay, Expressing, Inhibition, Migration, Membrane

Journal: Reviews in Cardiovascular Medicine

Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

doi: 10.31083/j.rcm2407214

Figure Lengend Snippet: The lncRNA associated ceRNA networks of septic cardiovascular toxicity in non-cardiomyocytes .

Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

Techniques: Biomarker Discovery, Expressing

Journal: Reviews in Cardiovascular Medicine

Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

doi: 10.31083/j.rcm2407214

Figure Lengend Snippet: LncRNA-mediated competitive endogenous RNA networks in septic cardiomyopathy . LncRNAs CRNDE, MIAT , and SNHG1 are associated with septic cardiomyopathy by regulating oxidative stress. LncRNAs H19, RMRP , and TTN-AS1 are associated with septic cardiomyopathy by regulating mitochondrial injury. Yin Yang-1 and matrine alleviate septic cardiomyopathy by upregulating LINC00472 and downregulating PTENP1 , respectively. Palmitic acid and saturated fatty acid aggravate septic cardiomyopathy by upregulating GAS5 and MALAT1 , respectively. The up-arrow indicates upregulation and the down-arrow indicates downregulation. The arrow-line represents promotion and the T-line represents inhibition. ANRIL, antisense ncRNA in the INK4 locus; CRNDE , colorectal neoplasia differentially expressed; E2F2, E2F transcription factor 2; GAS5 , growth arrest specific 5; H19 , H19 imprinted maternally expressed transcript; HMGB1, high mobility group protein 1; HSPA4, heat shock 70 kDa protein 4; IL-6, interleukin-6; MALAT1 , metastasis associated lung adenocarcinoma transcript 1; MAOA, monoamine oxidase A; MIAT, myocardial infarction associated transcript; NF- κ B, nuclear factor- κ B; PTENP1 , PTEN pseudogene-1; RMRP , RNA component of mitochondrial RNA processing endoribonuclease; SIRT1, sirtuins 1; SNHG16 , small nucleolar RNA host gene 16; SORBS2, sorbin and SH3 domain‐containing 2; TLR4, toll-like receptor 4; TRAF6, TNF receptor associated factor; TTN-AS1 , TTN antisense RNA 1; XIAP, X-linked inhibitor of apoptosis gene; lncRNA, long non-coding RNA.

Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

Techniques: Inhibition

Journal: Reviews in Cardiovascular Medicine

Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

doi: 10.31083/j.rcm2407214

Figure Lengend Snippet: LncRNA-mediated ceRNA networks in vascular endothelial cells and mononuclear macrophages under sepsis environment . LncRNAs LUADT1, HULC , and SNHG15 are involved in sepsis endothelial dysfunction. LncRNAs PVT1, HOTAR , and SNHG16 are involved in sepsis-induced macrophage polarization. In general, lncRNA MALAT1 aggravates sepsis-induced endothelial dysfunction and macrophage polarization, but MALAT1 inhibits LPS-induced RAW264.7 macrophage apoptosis by regulating has-miR-346/SMAD3 axis. The up-arrow indicates upregulation and the down-arrow indicates downregulation. The arrow-line represents promotion and the T-line represents inhibition. HMGB1, high mobility group protein 1; HOTAIR , hox transcript antisense RNA; HULC, highly upregulated in liver cancer; IL-6, interleukin-6; LUADT1, lung adenocarcinoma transcript 1; MALAT1 , metastasis associated lung adenocarcinoma transcript 1; MCEMP1, mast cell expressed membrane protein 1; NF- κ B, nuclear factor- κ B; PIM-1, pim-1 proto-oncogene; PVT1 , plasmacytoma variant translocation 1; SMAD3, SMAD family member 3; SNHG15/16 , small nucleolar RNA host gene 15/16; TLR4, toll-like receptor 4; TRPM7, transient receptor potential melastatin-subfamily member 7; lncRNA, long non-coding RNA.

Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

Techniques: Inhibition, Membrane, Variant Assay, Translocation Assay

Journal: Reviews in Cardiovascular Medicine

Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

doi: 10.31083/j.rcm2407214

Figure Lengend Snippet: Apoptosis and pyroptosis associated competitive endogenous RNA networks in septic cardiomyopathy . LncRNAs CYTOR, GAS5, KCNQ1OT1, LUCAT1 , and NEAT1 are associated with septic cardiomyopathy by regulating apoptosis. LncRNAs XIST and ZFAS1 are associated with septic cardiomyopathy by regulating pyroptosis. The up-arrow indicates upregulation and the down-arrow indicates downregulation. The arrow-line represents promotion and the T-line represents inhibition. c-Fos, A nuclear phosphoprotein; CYTOR , cytoskeleton regulator RNA; GAS5 , growth arrest specific 5; KCNQ1OT1 , KCNQ1 opposite strand/antisense transcript 1; LUCAT1 , lung cancer-related transcript 1; NEAT1 , nuclear paraspeckle assembly transcript 1; NF- κ B, nuclear factor- κ B; NLRP3, NOD-like receptor thermal protein domain associated protein 3; PGC-1α, peroxisome proliIerators-activated receptor γ coactivator l alpha; ROCK1, rho associated coiled-coil containing protein kinase 1; SESN2, sestrin 2; SIRT1, silent information regulator 1; XIAP, X-linked inhibitor of apoptosis gene; XIST , X-inactive specific transcript; ZFAS1 , zinc finger antisense 1; lncRNA, long non-coding RNA.

Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

Techniques: Inhibition

Journal: Reviews in Cardiovascular Medicine

Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

doi: 10.31083/j.rcm2407214

Figure Lengend Snippet: The lncRNA- and circRNA-associated competitive endogenous RNA networks in septic cardiovascular dysfunction . The regulatory mechanisms and potential functions of ceRNA networks in septic cardiomyopathy and vascular paralysis by regulating inflammation, oxidative response, endothelial dysfunction, macrophage polarization, apoptosis and pyroptosis, along with metabolic energy impairment and endoplasmic reticulum stress. See text for details.

Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

Techniques:

Journal: Journal of Advanced Research

Article Title: LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1

doi: 10.1016/j.jare.2023.01.017

Figure Lengend Snippet: Schematic diagram of the signalling pathways regulated by NIPA1-SO . Our study has uncovered an athero-protective role of the lncRNA NIPA1-SO , which, by interacting with a single transcription factor, is capable of inhibiting monocyte adhesion and foam cell formation, two fundamental processes in atherosclerosis. The transcription factor FUBP1 negatively regulates NIPA1 expression; this inhibitory effect is increased by the interaction of NIPA1-SO with FUBP1, resulting in lower transcription of NIPA1. A reduction in NIPA1 protein results in increased BMPR2 protein due to a reduction in NIPA1-mediated BMPR2 endocytosis and degradation, leading to higher levels of Smad1/5/8 phosphorylation (pSmad1/5/8), which, through complexation with Smad4, inhibit transcription of the adhesion molecules VCAM1 and ICAM1, reducing monocyte adhesion to endothelial cells. Further, the pSmad1/5/8:Smad4 complex promotes ABCA1 and ABCG1 transcription, both of which promote cholesterol efflux via high-density lipoprotein (HDL) particles, thereby inhibiting foam cell formation.

Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8 × 60 K, Arraystar) and scanned using Agilent Scanner G2505C.

Techniques: Expressing, Phospho-proteomics

Journal: OncoTargets and therapy

Article Title: Expression and clinical significance of the long non-coding RNA PVT1 in human gastric cancer

doi: 10.2147/OTT.S68854

Figure Lengend Snippet: Cluster diagram of lncRNA expression data in gastric cancer and corresponding non-tumor tissues detected by the Agilent Human LncRNA 4×180 K Expression Microarray (Agilent Technologies Inc., Santa Clara, CA, USA). Abbreviation: lncRNA, long-non-coding RNA.

Article Snippet: The labeled complementary DNA was hybridized to the Agilent Human LncRNA 4×180 K Expression Microarray (Agilent Technologies Inc., Santa Clara, CA, USA), which contains 37,000 human lncRNAs and 34,000 mRNA probes (CapitalBio Corporation).

Techniques: Expressing, Microarray